Manufacturer: 

Discreption

Zena Max SARS-CoV-2 Direct qPCR assay system is a Real Time PCR that enables the direct amplification of COVID-19 infection in human samples like nasopharyngeal swabs (NPS), nasal swabs, nasal wash or aspirate or bronchoalveolar lavage (BAL) using one-step RT-qPCR by first reverse transcribing the genomic RNA target to cDNA, followed by amplification of the assay target and detection by the hydrolysis probe method of qPCR.

 

Technical Specifications

Features

  • Rapid detection of the Novel SARS-CoV-2 (Covid-19) in around 1 hour
  • Protocol that does not require RNA extraction.
  • Detects 2 target genes in two independent channels (ORF1ab / N)
  • Positive internal control to confirm RNA quality on a third independent channel.
  • The Zena Max COVID-19 direct qPCR kit has a sensitivity of 10 copies per reaction and 100% specificity.
  • Compatible with standard qPCR machines (Rotor Gene Q, Lightcycler, Biorad CFX96 etc.)
  • Low sample volume requirements
  • No additional specialist instrumentation; testing carried out on standard qPCR machines
  • Internal positive control to confirm RNA quality is acceptable for diagnostic testing
  • Stable at -20 °C for up to 18 months

Format

  • 100 or 600 reactions

Shelf life

  • 18 months

Sample

  • Aspirate, BAL, swab

Test time

  • 75 min