Manufacturer: 

Description

The PCR kit is designed to detect C677T mutation in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymerase Chain Reaction (PCR). The method is based on amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide cytosine/thymine polymorphism in site 677 (C677T). Presence of wild-type allele (C677C) is detected in the FAM fluorescent channel and mutant allele (T677T) in the HEX fluorescent channel. In case of heterozygous genotype (C677T) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the “hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostic.

 

 

Technical Features

Specifications:

  • Target Sequence: C677T mutation in the gene for methylenetetrahydrofolate reductase (MTHFR)
  • Sensitivity: Reaches up to 2 ng/µl
  • Diagnostic Specificity: 100 %
  • Diagnostic Sensitivity :100 %
  • Quality Control In accordance with ISO 13485, each lot of GeneProof PCR Kit is tested against predetermined specifications to ensure consistent product quality.
  • External Quality Assessment Regularly tested by Instand e.V. External Quality Assessment panels
  • Required Detection Channels FAM, HEX
  • Compatible  with a wide range of Real-time  PCR devices (LightCycler® 2.0 / 480, Applied Biosystems 7300 / 7500 Real-Time PCR System, Rotor-Gene 3000 / 6000 κα)
  • Stable at -20 ± 5 °C
  • Kit is stable after 15 repeated freezing/thawing cycles
    CE-IVD certified diagnostic test

Format

  • 25/50/100 reactions

Shelf life

  • 18 months from production date

Sample

  • Whole blood

Test time

  • 50 min

Results